Stable isotope probing with 18O-water to investigate microbial growth and death in environmental samples.
نویسندگان
چکیده
Growth and mortality of microorganisms have been characterized through DNA stable isotope probing (SIP) with 18O-water in soils from a range of ecosystems. Conventional SIP has been improved by sequencing a marker gene in all fractions retrieved from an ultracentrifuge tube to produce taxon density curves, which allow estimating the atom percent isotope composition of each microbial taxon's genome. Very recent advances in SIP with 18O-water include expansion of the technique to aquatic samples, investigations of microbial turnover in soil, and the first use of 18O-water in RNA-SIP studies.
منابع مشابه
Validation of heavy-water stable isotope probing for the characterization of rapidly responding soil bacteria.
Rapid responses of bacteria to sudden changes in their environment can have important implications for the structure and function of microbial communities. In this study, we used heavy-water stable isotope probing (H2(18)O-SIP) to identify bacteria that respond to soil rewetting. First, we conducted experiments to address uncertainties regarding the H2(18)O-SIP method. Using liquid chromatograp...
متن کاملIsotope-hydrochemistry of Arnave karstic spring and Shirin dareh dam reservoir, North Khorasan
Surface and groundwater resources such as Arnaveh karstic spring and Shirin Dareh reservoir are the main sources of drinking water and agricultural activity in the North Khorasan province, northeast of Iran. The main agents of this study are to evaluate the origin, hydrochemical and isotope characteristics of water resources and also suitability of the Shirin dareh reservoir water for drinking ...
متن کاملCharacterization of growing microorganisms in soil by stable isotope probing with H218O.
A new approach to characterize growing microorganisms in environmental samples based on labeling microbial DNA with H(2)(18)O is described. To test if sufficient amounts of (18)O could be incorporated into DNA to use water as a labeling substrate for stable isotope probing, Escherichia coli DNA was labeled by cultivating bacteria in Luria broth with H(2)(18)O and labeled DNA was separated from ...
متن کاملStable isotope ratios and forensic analysis of microorganisms.
In the aftermath of the anthrax letters of 2001, researchers have been exploring various analytical signatures for the purpose of characterizing the production environment of microorganisms. One such signature is stable isotope ratios, which in heterotrophs, are a function of nutrient and water sources. Here we discuss the use of stable isotope ratios in microbial forensics, using as a database...
متن کاملEstimating taxon-specific population dynamics in diverse microbial communities
Understanding how population-level dynamics contribute to ecosystem-level processes is a primary focus of ecological research and has led to important breakthroughs in the ecology of macroscopic organisms. However, the inability to measure population-specific rates, such as growth, for microbial taxa within natural assemblages has limited ecologists’ understanding of how microbial populations i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Current opinion in biotechnology
دوره 41 شماره
صفحات -
تاریخ انتشار 2016